Summary
Catalog No:A1229
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - PRKAA1 antibody (A1229)

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Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-300 of human PRKAA1 (NP_006242.5).
SequenceMRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISL
Gene ID5562
Swiss ProtQ13131
SynonymsAMPK, AMPKa1
Calculated MW64kDa/65kDa
Observed MW65kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHT-29, HeLa, HepG2, U-251MG, Mouse spleen, Mouse lung, Mouse kidney, Rat heart
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (HeLa, Human, Rat)

PRKAA1 Polyclonal Antibody images

ABclonal:Western blot - PRKAA1 antibody (A1229)

Western blot - PRKAA1 antibody (A1229)

Western blot analysis of extracts of various cell lines, using PRKAA1 antibody (A1229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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