Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

PPARα Mouse mAb (A10183)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - PPARα Mouse mAb (A10183)

Western blot analysis of various lysates using PPARα Mouse mAb (A10183) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name PPARα Mouse mAb
Catalog No. A10183
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.

Immunogen information

Immunogen Recombinant protein of human PPARα
Sequence Email for sequence
Gene ID 5465
Swiss prot Q07869
Synonyms PPAR; NR1C1; hPPAR; PPARalpha; PPAR-alpha; PPARα
Calculated MW 52kDa
Observed MW 52kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:10 - 1:25
  • IF/ICC 1:10 - 1:25
  • FC 1:10 - 1:25
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Flow Cytometry    
Positive samples Mouse liver, Mouse kidney
Cellular location Nucleus
Customer validation

WB (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

PPARα Mouse mAb images

ABclonal:Western blot - PPARα Mouse mAb (A10183)}

Western blot - PPARα Mouse mAb (A10183)

Western blot analysis of various lysates using PPARα Mouse mAb (A10183) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PPARA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PPARA. (Distance between topics and target gene indicate popularity.) PPARA

* Data provided by citexs.com, for reference only.

Publishing research using A10183? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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