Summary
Catalog No:A7920
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - POU5F1 antibody (A7920)
ABclonal:Immunohistochemistry  - POU5F1 antibody (A7920)

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Background

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Immunogen information

ImmunogenA synthetic peptide of human POU5F1
SequenceEmail for sequence
Gene ID5460
Swiss ProtQ01860
SynonymsOct-3, Oct-4, OCT3, OCT4, OTF-3, OTF3, OTF4
Calculated MW30kDa/38kDa
Observed MW50kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples
Cellular LocationCytoplasm, Nucleus
Customer Validation

IHC (Human)

WB (Human)

POU5F1 Polyclonal Antibody images

ABclonal:Western blot - POU5F1 antibody (A7920)

Western blot - POU5F1 antibody (A7920)

Western blot analysis of extracts of various cell lines, using POU5F1 antibody (A7920) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - POU5F1 antibody (A7920)

Immunohistochemistry - POU5F1 antibody (A7920)

Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody (A7920) at dilution of 1:100 (40x lens).

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