Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PHGDH Rabbit pAb (A4617)

Review (1) Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - PHGDH Rabbit pAb (A4617)

Western blot analysis of various lysates using PHGDH Rabbit pAb (A4617) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Review
ABclonal: review for PHGDH Rabbit pAb(A4617)

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Overview

Product name PHGDH Rabbit pAb
Catalog No. A4617
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Immunogen information

Immunogen A synthetic peptide of human PHGDH
Sequence Email for sequence
Gene ID 26227
Swiss prot O43175
Synonyms NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113; PHGDH
Calculated MW 57kDa
Observed MW 57kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Flow Cytometry    
Positive samples B cells, LO2
Cellular location cytosol, extracellular exosome
Customer validation

WB (HepG2Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PHGDH Rabbit pAb images

ABclonal:Western blot - PHGDH Rabbit pAb (A4617)}

Western blot - PHGDH Rabbit pAb (A4617)

Western blot analysis of various lysates using PHGDH Rabbit pAb (A4617) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PHGDH. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PHGDH. (Distance between topics and target gene indicate popularity.) PHGDH

* Data provided by citexs.com, for reference only.

Publishing research using A4617? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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