Summary
Catalog No:A0264
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

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Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1).
SequenceMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKI
Gene ID5111
Swiss ProtP12004
SynonymsATLD2
Calculated MW28kDa
Observed MW36kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples
Cellular LocationNucleus
Customer Validation

IHC (Human)

WB (Mouse, Human)

PCNA Polyclonal Antibody images

ABclonal:Western blot - PCNA antibody (A0264)

Western blot - PCNA antibody (A0264)

Western blot analysis of extracts of various cell lines, using PCNA antibody (A0264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded human colon using PCNA antibody (A0264) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded human gastric using PCNA antibody (A0264) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse lung using PCNA antibody (A0264) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse liver using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PCNA antibody (A0264)

Immunohistochemistry - PCNA antibody (A0264)

Immunohistochemistry of paraffin-embedded mouse ovary using PCNA antibody (A0264) at dilution of 1:100 (40x lens).

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