Summary
Catalog No:A0942
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - PARP1 antibody (A0942)
ABclonal:Immunohistochemistry  - PARP1 Antibody (A0942)
ABclonal:Immunohistochemistry  - PARP1 Antibody (A0942)
ABclonal:Immunohistochemistry  - PARP1 Antibody (A0942)

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Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
SequenceDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVT
Gene ID142
Swiss ProtP09874
SynonymsADPRT, ADPRT1, ADPRT1, ARTD1, pADPRT-1, PARP, PARP-1, PPOL
Calculated MW113kDa
Observed MW115kDa

Applications

ReactivityHuman, Rat
Tested applicationsWB, IHC
Recommended DilutionWB 1:1000 - 1:3000
IHC 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, SKOV3, HeLa, Jurkat, HepG2
Cellular LocationNucleus, nucleolus
Customer Validation

WB (HCC827)

PARP1 Polyclonal Antibody images

ABclonal:Western blot - PARP1 antibody (A0942)

Western blot - PARP1 antibody (A0942)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0942) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - PARP1 Antibody (A0942)

Immunohistochemistry - PARP1 Antibody (A0942)

Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PARP1 Antibody (A0942)

Immunohistochemistry - PARP1 Antibody (A0942)

Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - PARP1 Antibody (A0942)

Immunohistochemistry - PARP1 Antibody (A0942)

Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibody (A0942) at dilution of 1:100 (40x lens).

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