Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

OSTM1 Rabbit pAb (A11685)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - OSTM1 Rabbit pAb (A11685)

Western blot analysis of various lysates using OSTM1 Rabbit pAb (A11685) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name OSTM1 Rabbit pAb
Catalog No. A11685
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 32-284 of human OSTM1 (NP_054747.2).
Sequence ALPFGSSPHRVFHDLLSEQQLLEVEDLSLSLLQGGGLGPLSLPPDLPDLDPECRELLLDFANSSAELTGCLVRSARPVRLCQTCYPLFQQVVSKMDNISRAAGNTSESQSCARSLLMADRMQIVVILSEFFNTTWQEANCANCLTNNSEELSNSTVYFLNLFNHTLTCFEHNLQGNAHSLLQTKNYSEVCKNCREAYKTLSSLYSEMQKMNELENKAEPGTHLCIDVEDAMNITRKLWSRTFNCSVPCSDTVP
Gene ID 28962
Swiss prot Q86WC4
Synonyms GL; GIPN; OPTB5; HSPC019; OSTM1
Calculated MW 37kDa
Observed MW 30-37kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, 293T, A-549, Raji, B cells, Mouse brain, Mouse kidney
Cellular location Lysosome membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Research Area

OSTM1 Rabbit pAb images

ABclonal:Western blot - OSTM1 Rabbit pAb (A11685)}

Western blot - OSTM1 Rabbit pAb (A11685)

Western blot analysis of various lysates using OSTM1 Rabbit pAb (A11685) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on OSTM1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to OSTM1. (Distance between topics and target gene indicate popularity.) OSTM1

* Data provided by citexs.com, for reference only.

Publishing research using A11685? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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