Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NRAS Mouse mAb (A11851)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - NRAS Mouse mAb (A11851)

Western blot analysis of various lysates using NRAS Mouse mAb (A11851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name NRAS Mouse mAb
Catalog No. A11851
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.

Immunogen information

Immunogen A synthetic peptide of human NRAS
Sequence Email for sequence
Gene ID 4893
Swiss prot P01111
Synonyms NS6; CMNS; KRAS; NCMS; ALPS4; N-ras; NRAS1; NRAS
Calculated MW 21kDa
Observed MW 21kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:500
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples A-549, 293T
Cellular location Cell membrane, Cytoplasmic side, Golgi apparatus membrane, Lipid-anchor

Documents

Certificate of Compliance

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Lot number

Research Area

NRAS Mouse mAb images

ABclonal:Western blot - NRAS Mouse mAb (A11851)}

Western blot - NRAS Mouse mAb (A11851)

Western blot analysis of various lysates using NRAS Mouse mAb (A11851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on NRAS. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NRAS. (Distance between topics and target gene indicate popularity.) NRAS

* Data provided by citexs.com, for reference only.

Publishing research using A11851? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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