Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NONO/p54nrb Rabbit pAb (A5282)

Review (1) Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NONO/p54nrb Rabbit pAb (A5282)

Western blot analysis of extracts of various cell lines, using NONO/p54nrb antibody (A5282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded rat lung using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded rat liver using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded mouse brain using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)

Immunofluorescence analysis of C6 cells using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)

Immunofluorescence analysis of NIH-3T3 cells using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)

Immunofluorescence analysis of U-2 OS cells using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for NONO/p54nrb Rabbit pAb(A5282)

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Overview

Product name NONO/p54nrb Rabbit pAb
Catalog No. A5282
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO/p54nrb (NP_001138881.1).
Sequence MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEM
Gene ID 4841
Swiss prot Q15233
Synonyms P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114; NONO/p54nrb
Calculated MW 54kDa
Observed MW 58kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples BxPC-3, 293T, Mouse brain
Cellular location Nucleus, Nucleus speckle, nucleolus
Customer validation

WB (Human cellsMus musculus)

IF (Homo sapiens, Mus musculus)

IP (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

NONO/p54nrb Rabbit pAb images

ABclonal:Western blot - NONO/p54nrb Rabbit pAb (A5282)}

Western blot - NONO/p54nrb Rabbit pAb (A5282)

Western blot analysis of extracts of various cell lines, using NONO/p54nrb antibody (A5282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)}

Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded rat lung using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)}

Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded rat liver using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)}

Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)}

Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)}

Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded mouse brain using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)}

Immunohistochemistry - NONO/p54nrb Rabbit pAb (A5282)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)}

Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)

Immunofluorescence analysis of C6 cells using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)}

Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)

Immunofluorescence analysis of NIH-3T3 cells using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)}

Immunofluorescence - NONO/p54nrb Rabbit pAb (A5282)

Immunofluorescence analysis of U-2 OS cells using NONO/p54nrb Rabbit pAb (A5282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on NONO. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NONO. (Distance between topics and target gene indicate popularity.) NONO

* Data provided by citexs.com, for reference only.

Publishing research using A5282? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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