Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NME1/NM23A Mouse mAb (A0087)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - NME1/NM23A Mouse mAb (A0087)

Western blot analysis of various lysates using NME1/NM23A Mouse mAb (A0087).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name NME1/NM23A Mouse mAb
Catalog No. A0087
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Immunogen information

Immunogen A synthetic peptide of human NME1/NM23A
Sequence Email for sequence
Gene ID 4830
Swiss prot P15531
Synonyms NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1; NME1/NM23A
Calculated MW 17kDa
Observed MW 17kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples HepG2, Jurkat, Colo320, MCF7
Cellular location Cytoplasm, Nucleus

Research Area

NME1/NM23A Mouse mAb images

ABclonal:Western blot - NME1/NM23A Mouse mAb (A0087)}

Western blot - NME1/NM23A Mouse mAb (A0087)

Western blot analysis of various lysates using NME1/NM23A Mouse mAb (A0087).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on NME1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NME1. (Distance between topics and target gene indicate popularity.) NME1

* Data provided by citexs.com, for reference only.

Publishing research using A0087? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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