Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NLK Mouse mAb (A11852)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - NLK Mouse mAb (A11852)

Western blot analysis of various lysates using NLK Mouse mAb (A11852) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name NLK Mouse mAb
Catalog No. A11852
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Enables ubiquitin protein ligase binding activity. Involved in protein stabilization and transforming growth factor beta receptor signaling pathway. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in cytoplasm and nucleus.

Immunogen information

Immunogen Recombinant protein of human NLK
Sequence Email for sequence
Gene ID 51701
Swiss prot Q9UBE8
Synonyms NLK
Calculated MW 58kDa
Observed MW 65kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples HepG2, HeLa, A-431
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

NLK Mouse mAb images

ABclonal:Western blot - NLK Mouse mAb (A11852)}

Western blot - NLK Mouse mAb (A11852)

Western blot analysis of various lysates using NLK Mouse mAb (A11852) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on NLK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NLK. (Distance between topics and target gene indicate popularity.) NLK

* Data provided by citexs.com, for reference only.

Publishing research using A11852? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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