Summary
Catalog No:A1187
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - NFKBIA antibody (A1187)

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Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1).
SequenceMFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQ
Gene ID4792
Swiss ProtP25963
SynonymsIKBA, MAD-3, NFKBI
Calculated MW35kDa
Observed MW38kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesDU145, Mouse thymus, Rat thymus
Cellular LocationCytoplasm, Nucleus
Customer Validation

IHC (Human glioma)

WB (Hela, HepG2, Mouse splenocytes, Human)

IF (Human, Mouse)

NFKBIA Polyclonal Antibody images

ABclonal:Western blot - NFKBIA antibody (A1187)

Western blot - NFKBIA antibody (A1187)

Western blot analysis of extracts of various cell lines, using NFKBIA antibody (A1187) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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