Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

NRF2 Rabbit mAb (A11891)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Monkey

ABclonal:Western blot - NRF2 Rabbit mAb (A11891)

Western blot analysis of extracts of various cell lines, using NRF2 antibody (A11891).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name NRF2 Rabbit mAb
Catalog No. A11891
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Immunogen information

Immunogen Recombinant protein of human NRF2
Sequence Email for sequence
Gene ID 4780
Swiss prot Q16236
Synonyms NRF2; HEBP1; Nrf-2; IMDDHH
Calculated MW 68kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Monkey
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 1:20 - 1:50
  • FC 1:20 - 1:50
  • ChIP 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    Flow Cytometry    
Positive samples MEF(WT), U2OS
Cellular location Cytoplasm, Nucleus, cytosol

Research Area

NRF2 Rabbit mAb images

ABclonal:Western blot - NRF2 Rabbit mAb (A11891)}

Western blot - NRF2 Rabbit mAb (A11891)

Western blot analysis of extracts of various cell lines, using NRF2 antibody (A11891).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on NFE2L2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NFE2L2. (Distance between topics and target gene indicate popularity.) NFE2L2

* Data provided by citexs.com, for reference only.

Publishing research using A11891? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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