Summary
Catalog No:A0484
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - NES antibody (A0484)

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Background

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.

Immunogen information

ImmunogenA synthetic peptide of human NES
SequenceEmail for sequence
Gene ID10763
Swiss ProtP48681
SynonymsNbla00170
Calculated MW177kDa
Observed MW300kDa

Applications

ReactivityHuman
Tested applicationsWB, IHC, IF, FC
Recommended DilutionWB 1:500 - 1:1000
IHC 1:20 - 1:50
IF 1:20 - 1:50
FC 1:20 - 1:50
Storage BufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Flow Cytometry    
Positive samplesSH-SY5Y
Cellular Location
Customer Validation

IHC (Rat)

WB (Human)

IF (Human)

NES Polyclonal Antibody images

ABclonal:Western blot - NES antibody (A0484)

Western blot - NES antibody (A0484)

Western blot analysis of extracts of SH-SY5Y cells, using NES antibody (A0484).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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