Product Type > Antibodies > Tag and Loading Control Antibodies

Actin (plant specific) Mouse mAb (AC009)

Review (1) Publications (64) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Various Plants

ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)

Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (AC009).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Actin (plant specific) Mouse mAb (AC009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for Actin (plant specific) Mouse mAb(AC009)

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Overview

Product name Actin (plant specific) Mouse mAb
Catalog No. AC009
Host species Mouse
Purification method Affinity purification
Isotype IgG1
CloneNo. AMC0496

Background

Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen information

Immunogen A synthetic peptide corresponding to Actin (plant specific).
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW 45kDa
Observed MW 45kDa

Applications

Reactivity Various Plants
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Arabidopsis thaliana tissue, Arabidopsis thaliana, Oryza sativa, Cassava, Zea mays
Cellular location
Customer validation

WB (Zea mays,Arabidopsis thaliana,TomatoBrassica napus, Arabidopsis thaliana, Populus L., Populus davidiana×P.bolleana, Populus trichocarpa Torr. & Gray, Homo sapiens, Oryza sativa, N. benthamiana plants, Nicotiana tabacum, Lycopersicon esculentum, Zea mays, Glycine max L, Triticum aestivum, Mus musculus, Solanum tuberosum, Nicotiana benthamiana, N. benthamiana, Paeonia suffruticosa, , Other)

SB (Zea mays)

Co-IP (Arabidopsis thaliana)

IB (Coniothyrium minitans)

ChIP (Trifoliata orange)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Actin (plant specific) Mouse mAb images

ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)}

Western blot - Actin (plant specific) Mouse mAb (AC009)

Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (AC009).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)}

Western blot - Actin (plant specific) Mouse mAb (AC009)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Actin (plant specific) Mouse mAb (AC009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC009? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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