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Mouse anti plant actin Monoclonal Antibody

Summary
Catalog No:AC009
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - Plants actin antibody (AC009)
ABclonal:Western blot - plants actin antibody (AC009)

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Background

Immunogen information

ImmunogenRecombinant Protein of plants actin
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW45kDa
Observed MW45kDa

Applications

ReactivityVarious Plants
Tested applicationsWB
Recommended DilutionWB 1:1000 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesArabidopsis thaliana, Oryza sativa, Cassava, Zea mays
Cellular Location
Customer Validation

WB (Zea mays,Arabidopsis thaliana,Tomato, Brassica napus, Poplar)

Mouse anti plant actin Monoclonal Antibody images

ABclonal:Western blot - Plants actin antibody (AC009)

Western blot - Plants actin antibody (AC009)

Western blot analysis of extracts of various cell lines, using Plants actin antibody (AC009) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - plants actin antibody (AC009)

Western blot - plants actin antibody (AC009)

Western blot analysis of lysates from Arabidopsis thaliana tissue using plants actin antibody (AC009).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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