Product Type > Antibodies > Tag and Loading Control Antibodies

Mouse anti TAP-Tag mAb (AE021)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - Mouse anti TAP-Tag mAb (AE021)

Western blot analysis of recombinant TAP protein using mouse anti TAP-Tag mAb (AE021) at dilution of 1:5000.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Mouse anti TAP-Tag mAb
Catalog No. AE021
Host species Mouse
Purification method Affinity purification
Isotype IgG1
CloneNo. AMC0510

Background

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Immunogen information

Immunogen A synthetic peptide of TAP-Tag
Sequence CSSGALDYDIPTTASENLYFQ
Gene ID
Swiss prot
Synonyms TAP; TAP tag; TAP-tag
Calculated MW
Observed MW Refer to Figures

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples
Cellular location

Mouse anti TAP-Tag mAb images

ABclonal:Western blot - Mouse anti TAP-Tag mAb (AE021)}

Western blot - Mouse anti TAP-Tag mAb (AE021)

Western blot analysis of recombinant TAP protein using mouse anti TAP-Tag mAb (AE021) at dilution of 1:5000.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on TAP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TAP. (Distance between topics and target gene indicate popularity.) TAP

* Data provided by citexs.com, for reference only.

Publishing research using AE021? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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