Overview
Product name
Mouse anti RFP-Tag mAb
Catalog No.
AE020
Host species
Mouse
Purification method
Affinity purification
Isotype
IgG1
CloneNo.
AMC0509
Background
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Immunogen information
Immunogen
Recombinant protein of RFP-Tag
Sequence
MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHHLFL
Gene ID
Swiss prot
Synonyms
RFP; RFP tag; RFP-tag
Calculated MW
27kDa
Observed MW
Refer to Figures
Applications
Reactivity
Species independent
Tested applications
WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
Storage buffer
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key
Western blotting
Positive samples
Cellular location
Customer validation
WB (Helicoverpa armigera, Cotton bollworms, Nicotiana benthamiana, Oryza sativa, Homo sapiens, Saccharomyces cerevisiae, Other)
IP (Gallus gallus)
Co-IP (Helicoverpa armigera, Penaeus japonicus, Other)
ChIP (Homo sapiens)
Mouse anti RFP-Tag mAb images
Western blot - Mouse anti RFP-Tag mAb (AE020)
Western blot analysis of recombinant RFP protein using RFP-tag antibody (AE020) at dilution of 1:10000. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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Related Targets
Based on existing publications, the following genes are closely related to research on RFP . (Numbers indicate number of published articles where
both targets appear.)
Related Research Area
Based on existing publications, the following research topics are related to RFP . (Distance between topics and target gene
indicate popularity.) RFP
* Data provided by citexs.com ,
for reference only.
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Autophagy
Application:
WB
IF:
16.016
Species:
Helicoverpa armigera
PMID:
Title:
Autophagy triggers CTSD (cathepsin D) maturation and localization inside cells to promote apoptosis
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
PLoS Genet
Application:
WB
IF:
5.91
Species:
Helicoverpa armigera
PMID:
Title:
Subunit P60 of phosphatidylinositol 3-kinase promotes cell proliferation or apoptosis depending on its phosphorylation status
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Development
Application:
WB
IF:
6.86
Species:
Cotton bollworms
PMID:
Title:
The homotetramerization of a GPCR transmits the 20-hydroxyecdysone signal and increases its entry into cells for insect metamorphosis
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
PLoS Pathog
Application:
WB
IF:
6.82
Species:
Nicotiana benthamiana
PMID:
Title:
Turnip mosaic virus P1 suppresses JA biosynthesis by degrading cpSRP54 that delivers AOCs onto the thylakoid membrane to facilitate viral infection
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Cell Mol Biol Lett
Application:
IP
IF:
5.787
Species:
Gallus gallus
PMID:
Title:
Esterase D stabilizes FKBP25 to suppress mTORC1
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Insect biochemistry and molecular biology
Application:
Co-IP
IF:
Species:
Helicoverpa armigera
PMID:
Title:
Juvenile hormone induces methoprene-tolerant 1 phosphorylation to increase interaction with Taiman in Helicoverpa armigera
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Plant physiology
Application:
WB
IF:
8
Species:
Oryza sativa
PMID:
Title:
Dual-function DEFENSIN 8 mediates phloem cadmium unloading and accumulation in rice grains
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
The Journal of biological chemistry
Application:
WB, ChIP
IF:
5.486
Species:
Homo sapiens
PMID:
Title:
The steroid hormone 20-hydroxyecdysone induces phosphorylation and aggregation of stromal interacting molecule 1 for store-operated calcium entry.
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
PLoS genetics
Application:
WB, Co-IP
IF:
5.917
Species:
Helicoverpa armigera
PMID:
Title:
The steroid hormone 20-hydroxyecdysone binds to dopamine receptor to repress lepidopteran insect feeding and promote pupation.
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Nature communications
Application:
WB
IF:
17.69
Species:
Saccharomyces cerevisiae
PMID:
Title:
Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat - PMC
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
International journal of biological macromolecules
Application:
Co-IP
IF:
8.02
Species:
Penaeus japonicus
PMID:
Title:
White spot syndrome virus hijacks host PP2A-FOXO axes to promote its propagation
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
Journal of nanobiotechnology
Application:
Co-IP
IF:
9.42
Species:
Other
PMID:
Title:
Non-transgenic, PAMAM co-delivery DNA of interactive proteins NbCRVP and NbCalB endows Nicotiana benthamiana with a stronger antiviral effect to RNA viruses - PMC
References for Mouse anti RFP-Tag mAb (AE020)
Product:
Mouse anti RFP-Tag mAb
Journal:
The Journal of biological chemistry
Application:
Co-IP, WB
IF:
0
Species:
Other
PMID:
Title:
KAT8 is upregulated and recruited to the promoter of Atg8 by FOXO to induce H4 acetylation for autophagy under 20-hydroxyecdysone regulation
Publishing research using AE020? Please let
us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic
purposes.