Summary
Catalog No:AE008
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - HA-tag antibody (AE008)
ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008)
ABclonal:Immunoprecipitation  - precipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (AE008)

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Background

Immunogen information

ImmunogenA synthetic peptide of YPYDVPDYA (HA-tag)-KLH
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to Figures

Applications

Reactivity
Tested applicationsWB, IF, IP
Recommended DilutionWB 1:2000 - 1:5000
IF 1:50 - 1:500
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular Location
Customer Validation

WB (unknow, Human, Mouse, Pig)

IP (Human)

IHC (Mouse)

CoIP (Oryza Sativa)

Mouse anti HA-Tag mAb images

ABclonal:Western blot - HA-tag antibody (AE008)

Western blot - HA-tag antibody (AE008)

Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody (AE008) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti HA-Tag mAb (AE008)

Immunofluorescence - Mouse anti HA-Tag mAb (AE008)

Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb (AE008). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation  - precipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (AE008)

Immunoprecipitation - precipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (AE008)

Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (AE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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