Summary
Catalog No:AE005
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - DDDDK-tag antibody (AE005)
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)
ABclonal:Immunoprecipitation  - precipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005)

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Background

Immunogen information

ImmunogenA synthetic Peptide of human DDDDK-Tag
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MWRefer to Figures

Applications

Reactivity
Tested applicationsWB, IHC, IF, IP
Recommended DilutionWB 1:2000 - 1:5000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular Location
Customer Validation

WB (Mouse, Human, Pig, Arabidopsis thaliana)

ChIP (Mouse)

CoIP (Oryza Sativa)

IF (Human)

Mouse anti DDDDK-Tag mAb images

ABclonal:Western blot - DDDDK-tag antibody (AE005)

Western blot - DDDDK-tag antibody (AE005)

Western blot analysis of over-expressed DDDDK-tagged protein in 293T cell using DDDDK-tag antibody (AE005) at different dilution. Each lane was loaded with 2 ug cell lysate.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence - Mouse anti DDDDK-Tag mAb (AE005)

Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (AE005). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation  - precipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005)

Immunoprecipitation - precipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (AE005). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

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