Summary
Catalog No:A5681
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - MSH3 antibody (A5681)

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Background

The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 617-761 of human MSH3 (NP_004696.2).
SequenceFNTSEEHHADMYRSDLPNPDTLSAELHCWRIKWKHRGKDIELPSTIYEALHLPDIKFFPNVYALLKVLCILPVMKVENERYENGRKRLKAYLRNTLTDQRSSNLALLNINFDIKHDLDLMVDTYIKLYTSKSELPTDNSETVENT
Gene ID4437
Swiss ProtP20585
SynonymsDUP, FAP4, MRP1
Calculated MW127kDa
Observed MW140kDa

Applications

ReactivityHuman
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSW620, HepG2, Jurkat
Cellular Location

MSH3 Polyclonal Antibody images

ABclonal:Western blot - MSH3 antibody (A5681)

Western blot - MSH3 antibody (A5681)

Western blot analysis of extracts of various cell lines, using MSH3 antibody (A5681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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