Catalog No:A1121
Host species:Rabbit
Purity:Affinity purification
ABclonal:Western blot - MSH2 antibody (A1121)
ABclonal:Immunoprecipitation - 3ug MSH2 antibody (A1121)

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This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
Gene ID4436
Swiss ProtP43246
Calculated MW97kDa/104kDa
Observed MW105kDa


ReactivityHuman, Mouse, Rat
Tested applicationsWB, IP
Recommended DilutionWB 1:500 - 1:2000
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samples
Cellular LocationNucleus

MSH2 Polyclonal Antibody images

ABclonal:Western blot - MSH2 antibody (A1121)

Western blot - MSH2 antibody (A1121)

Western blot analysis of extracts of various cell lines, using MSH2 antibody (A1121) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - 3ug MSH2 antibody (A1121)

Immunoprecipitation - 3ug MSH2 antibody (A1121)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (A1121). Western blot was performed from the immunoprecipitate using MSH2 antibody (A1121) at a dilition of 1:1000.

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