Summary
Catalog No:A1121
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - MSH2 antibody (A1121)
ABclonal:Immunoprecipitation - 3ug MSH2 antibody (A1121)

To buy,select size,quantity

Background

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
SequenceMAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNS
Gene ID4436
Swiss ProtP43246
SynonymsCOCA1, FCC1, HNPCC, HNPCC1, LCFS2
Calculated MW97kDa/104kDa
Observed MW105kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IP
Recommended DilutionWB 1:500 - 1:2000
IP 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samples
Cellular LocationNucleus

MSH2 Polyclonal Antibody images

ABclonal:Western blot - MSH2 antibody (A1121)

Western blot - MSH2 antibody (A1121)

Western blot analysis of extracts of various cell lines, using MSH2 antibody (A1121) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - 3ug MSH2 antibody (A1121)

Immunoprecipitation - 3ug MSH2 antibody (A1121)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (A1121). Western blot was performed from the immunoprecipitate using MSH2 antibody (A1121) at a dilition of 1:1000.

Submit an review for A1121

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Ask Us About This Product

submit your question on A1121 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

You Viewed