Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MRPL11 Rabbit pAb (A4945)

Review (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MRPL11 Rabbit pAb (A4945)

Western blot analysis of extracts of various cell lines, using MRPL11 antibody (A4945) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

Review
ABclonal: review for MRPL11 Rabbit pAb(A4945)

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Overview

Product name MRPL11 Rabbit pAb
Catalog No. A4945
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This nuclear gene encodes a 39S subunit component of the mitochondial ribosome. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene are found on chromosomes 5 and 12.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human MRPL11 (NP_057134.1).
Sequence MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAKK
Gene ID 65003
Swiss prot Q9Y3B7
Synonyms L11MT; CGI-113; MRP-L11; MRPL11
Calculated MW 21kDa
Observed MW 21kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HepG2, HeLa, MCF7, A-549, Mouse kidney, Mouse brain, Mouse heart, Rat heart
Cellular location Mitochondrion
Customer validation

WB (Human,Mouse)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

MRPL11 Rabbit pAb images

ABclonal:Western blot - MRPL11 Rabbit pAb (A4945)}

Western blot - MRPL11 Rabbit pAb (A4945)

Western blot analysis of extracts of various cell lines, using MRPL11 antibody (A4945) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MRPL11. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MRPL11. (Distance between topics and target gene indicate popularity.) MRPL11

* Data provided by citexs.com, for reference only.

Publishing research using A4945? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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