Summary
Catalog No:A2094
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - MKI67 antibody (A2094)
ABclonal:Immunofluorescence - MKI67 antibody (A2094)

To buy,select size,quantity

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Immunogen information

ImmunogenRecombinant protein of human MKI67
SequenceEmail for sequence
Gene ID4288
Swiss ProtP46013
SynonymsKIA, MIB-, MIB-1, PPP1R105
Calculated MW319kDa/358kDa
Observed MW360kDa

Applications

ReactivityHuman
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples
Cellular LocationChromosome, Nucleus, nucleolus
Customer Validation

IHC (Mouse)

IF (Mouse)

MKI67 Polyclonal Antibody images

ABclonal:Western blot - MKI67 antibody (A2094)

Western blot - MKI67 antibody (A2094)

Western blot analysis of extracts of HeLa cells, using MKI67 antibody (A2094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MKI67 antibody (A2094)

Immunofluorescence - MKI67 antibody (A2094)

Immunofluorescence analysis of HeLa cells using MKI67 antibody (A2094). Blue: DAPI for nuclear staining.

Submit an review for A2094

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Ask Us About This Product

submit your question on A2094 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A2094? Please let us know so that we can cite the reference in this datasheet.

You Viewed