Summary
Catalog No:A0288
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - MAPK8 antibody (A0288)
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)

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Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Immunogen information

ImmunogenRecombinant protein of human MAPK8
SequenceEmail for sequence
Gene ID5599
Swiss ProtP45983
SynonymsJNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
Calculated MW35kDa/44kDa/48kDa
Observed MW50kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB, IHC
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa, BT-474, Mouse liver
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (Mouse heart tissue, Rat, Human)

MAPK8 Polyclonal Antibody images

ABclonal:Western blot - MAPK8 antibody (A0288)

Western blot - MAPK8 antibody (A0288)

Western blot analysis of extracts of various cell lines, using MAPK8 antibody (A0288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)

Immunohistochemistry - MAPK8 antibody (A0288)

Immunohistochemistry of paraffin-embedded human gastric cancer using MAPK8 antibody (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)

Immunohistochemistry - MAPK8 antibody (A0288)

Immunohistochemistry of paraffin-embedded human lung cancer using MAPK8 antibody (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)

Immunohistochemistry - MAPK8 antibody (A0288)

Immunohistochemistry of paraffin-embedded human colon using MAPK8 antibody (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)

Immunohistochemistry - MAPK8 antibody (A0288)

Immunohistochemistry of paraffin-embedded human prostate using MAPK8 antibody (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAPK8 antibody (A0288)

Immunohistochemistry - MAPK8 antibody (A0288)

Immunohistochemistry of paraffin-embedded human stomach using MAPK8 antibody (A0288) at dilution of 1:100 (40x lens).

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