Summary
Catalog No:A0025
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - MAPK3 antibody (A0025)

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Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Immunogen information

ImmunogenA synthetic peptide of human MAPK3
SequenceEmail for sequence
Gene ID5595
Swiss ProtP27361
SynonymsERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM3
Calculated MW38kDa/40kDa/43kDa
Observed MW45kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:1000
Storage BufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, A-375, A-549
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (Human)

MAPK3 Monoclonal Antibody images

ABclonal:Western blot - MAPK3 antibody (A0025)

Western blot - MAPK3 antibody (A0025)

Western blot analysis of extracts of various cell lines, using MAPK3 antibody (A0025).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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