Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

ERK1 / ERK2 Mouse mAb (A10613)

Review (1) Publications (28) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ERK1 / ERK2 Mouse mAb (A10613)

Western blot analysis of various lysates, using ERK1 / ERK2 Mouse mAb (A10613) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Review
ABclonal: review for ERK1 / ERK2 Mouse mAb(A10613)

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Overview

Product name ERK1 / ERK2 Mouse mAb
Catalog No. A10613
Host species Mouse
Purification method Affinity purification
Isotype IgG
CloneNo. AMC0488

Background

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

Immunogen information

Immunogen A synthetic peptide of human ERK1 / ERK2
Sequence Email for sequence
Gene ID 55945595
Swiss prot P28482P27361
Synonyms
Calculated MW 36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW 42kDa/44kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, 293T, A-431, NIH/3T3, RAW 264.7, C6, PC12
Cellular location caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Customer validation

WB (mouse cellsHomo sapiens, Homo sapiens, Mus musculus, Rattus norvegicus)

IF (Mus musculus)

IHC (Mus musculus)

IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ERK1 / ERK2 Mouse mAb images

ABclonal:Western blot - ERK1 / ERK2 Mouse mAb (A10613)}

Western blot - ERK1 / ERK2 Mouse mAb (A10613)

Western blot analysis of various lysates, using ERK1 / ERK2 Mouse mAb (A10613) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK1. (Distance between topics and target gene indicate popularity.) MAPK1

* Data provided by citexs.com, for reference only.

Publishing research using A10613? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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