Products / Catalog Antibodies / Polyclonal Antibodies

MAP1LC3B Polyclonal Antibody

Summary
Catalog No:A7198
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - MAP1LC3B antibody (A7198)
ABclonal:Western blot - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)
ABclonal:Immunofluorescence - MAP1LC3B antibody (A7198)
ABclonal:Immunofluorescence - MAP1LC3B antibody (A7198)

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Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Immunogen information

ImmunogenA synthetic peptide of human MAP1LC3B
SequenceEmail for sequence
Gene ID81631
Swiss ProtQ9GZQ8
SynonymsATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
Calculated MW14kDa
Observed MW15kDa

Applications

ReactivityHuman, Mouse, Rat, Pig
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer Validation

WB (C2C12, Mouse cells, 293T, Rat cells, Human)

IHC (Squamous cell carcinoma)

IF (U87MG)

MAP1LC3B Polyclonal Antibody images

ABclonal:Western blot - MAP1LC3B antibody (A7198)

Western blot - MAP1LC3B antibody (A7198)

Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody (A7198) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - MAP1LC3B antibody (A7198)

Western blot - MAP1LC3B antibody (A7198)

Western blot analysis of extracts of pig liver, using MAP1LC3B antibody (A7198).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded rat heart using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded human lung cancer using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded mouse heart using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded human breast cancer using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - MAP1LC3B antibody (A7198)

Immunohistochemistry - MAP1LC3B antibody (A7198)

Immunohistochemistry of paraffin-embedded human stomach using MAP1LC3B antibody (A7198) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - MAP1LC3B antibody (A7198)

Immunofluorescence - MAP1LC3B antibody (A7198)

Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody (A7198) at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MAP1LC3B antibody (A7198)

Immunofluorescence - MAP1LC3B antibody (A7198)

Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody (A7198) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.

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