Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

MALT1 Rabbit pAb (A2144)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - MALT1 Rabbit pAb (A2144)

Western blot analysis of various lysates using MALT1 Rabbit pAb (A2144) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - MALT1 Rabbit pAb (A2144)

Immunofluorescence analysis of C6 cells using MALT1 Rabbit pAb (A2144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MALT1 Rabbit pAb (A2144)

Immunofluorescence analysis of L929 cells using MALT1 Rabbit pAb (A2144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MALT1 Rabbit pAb (A2144)

Immunofluorescence analysis of U-2 OS cells using MALT1 Rabbit pAb (A2144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name MALT1 Rabbit pAb
Catalog No. A2144
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a caspase-like protease that plays a role in BCL10-induced activation of NF-kappaB. The protein is a component of the CARMA1-BCL10-MALT1 (CBM) signalosome that triggers NF-kappaB signaling and lymphoctye activation following antigen-receptor stimulation. Mutations in this gene result in immunodeficiency 12 (IMD12). This gene has been found to be recurrently rearranged in chromosomal translocations with other genes in mucosa-associated lymphoid tissue lymphomas, including a t(11;18)(q21;q21) translocation with the baculoviral IAP repeat-containing protein 3 (also known as apoptosis inhibitor 2) locus [BIRC3(API2)-MALT1], and a t(14;18)(q32;q21) translocation with the immunoglobulin heavy chain locus (IGH-MALT1). Alternatively spliced transcript variants have been described for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 125-450 of human MALT1 (NP_776216.1).
Sequence PGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLL
Gene ID 10892
Swiss prot Q9UDY8
Synonyms MLT; MLT1; IMD12; PCASP1; MALT1
Calculated MW 92kDa
Observed MW 102kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, 293T
Cellular location Cytoplasm, Nucleus, perinuclear region
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

MALT1 Rabbit pAb images

ABclonal:Western blot - MALT1 Rabbit pAb (A2144)}

Western blot - MALT1 Rabbit pAb (A2144)

Western blot analysis of various lysates using MALT1 Rabbit pAb (A2144) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - MALT1 Rabbit pAb (A2144)}

Immunofluorescence - MALT1 Rabbit pAb (A2144)

Immunofluorescence analysis of C6 cells using MALT1 Rabbit pAb (A2144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MALT1 Rabbit pAb (A2144)}

Immunofluorescence - MALT1 Rabbit pAb (A2144)

Immunofluorescence analysis of L929 cells using MALT1 Rabbit pAb (A2144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MALT1 Rabbit pAb (A2144)}

Immunofluorescence - MALT1 Rabbit pAb (A2144)

Immunofluorescence analysis of U-2 OS cells using MALT1 Rabbit pAb (A2144) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MALT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MALT1. (Distance between topics and target gene indicate popularity.) MALT1

* Data provided by citexs.com, for reference only.

Publishing research using A2144? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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