Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

LTF Mouse mAb (A1597)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - LTF Mouse mAb (A1597)

Western blot analysis of extracts of various cell lines, using LTF antibody (A1597) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name LTF Mouse mAb
Catalog No. A1597
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Activity against both DNA and RNA viruses has been found, including activity against SARS-CoV-2, and HIV.

Immunogen information

Immunogen Recombinant protein of human LTF
Sequence Email for sequence
Gene ID 4057
Swiss prot P02788
Synonyms LF; HLF2; GIG12; HEL110; LTF
Calculated MW 78kDa
Observed MW 68kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples K-562, HeLa, 293T, Mouse lung, Mouse spleen, Rat lung
Cellular location Cytoplasm, Cytoplasmic granule, Nucleus, Secreted

Documents

Certificate of Compliance

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Lot number

Research Area

LTF Mouse mAb images

ABclonal:Western blot - LTF Mouse mAb (A1597)}

Western blot - LTF Mouse mAb (A1597)

Western blot analysis of extracts of various cell lines, using LTF antibody (A1597) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on LTF. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to LTF. (Distance between topics and target gene indicate popularity.) LTF

* Data provided by citexs.com, for reference only.

Publishing research using A1597? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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