Summary
Catalog No:A2582
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - LAMP1 antibody (A2582)
ABclonal:Immunohistochemistry  - LAMP1 antibody (A2582)

To buy,select size,quantity

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 80-280 of human LAMP1 (NP_005552.3).
SequenceCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHS
Gene ID3916
Swiss ProtP11279
SynonymsCD107a, LAMPA, LGP120
Calculated MW38kDa/44kDa
Observed MW120kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCell membrane, Endosome membrane, Late endosome, Lysosome membrane, Single-pass type I membrane protein
Customer Validation

WB (HEK293T, Human)

IF (Human)

LAMP1 Polyclonal Antibody images

ABclonal:Western blot - LAMP1 antibody (A2582)

Western blot - LAMP1 antibody (A2582)

Western blot analysis of extracts of various cell lines, using LAMP1 antibody (A2582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - LAMP1 antibody (A2582)

Immunohistochemistry - LAMP1 antibody (A2582)

Immunohistochemistry of paraffin-embedded mouse kidney using LAMP1 antibody (A2582) at dilution of 1:100 (40x lens).

Submit an review for A2582

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Ask Us About This Product

submit your question on A2582 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A2582? Please let us know so that we can cite the reference in this datasheet.

You Viewed