Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Cytokeratin 19 (KRT19) Rabbit mAb (A10948)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Cytokeratin 19 (KRT19) Rabbit mAb (A10948)

Western blot analysis of extracts of MCF-7 cells, using Cytokeratin 19 (Cytokeratin 19 (KRT19)) antibody (A10948).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Cytokeratin 19 (KRT19) Rabbit mAb
Catalog No. A10948
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

Immunogen information

Immunogen Recombinant protein of human Cytokeratin 19 (Cytokeratin 19 (KRT19))
Sequence Email for sequence
Gene ID 3880
Swiss prot P08727
Synonyms K19; CK19; K1CS; Cytokeratin 19 (KRT19)
Calculated MW 44kDa
Observed MW 44kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7
Cellular location apicolateral plasma membrane, costamere, cytoskeleton, cytosol, extracellular exosome, plasma membrane, sarcolemma, terminal web, Z disc

Research Area

Cytokeratin 19 (KRT19) Rabbit mAb images

ABclonal:Western blot - Cytokeratin 19 (KRT19) Rabbit mAb (A10948)}

Western blot - Cytokeratin 19 (KRT19) Rabbit mAb (A10948)

Western blot analysis of extracts of MCF-7 cells, using Cytokeratin 19 (Cytokeratin 19 (KRT19)) antibody (A10948).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on KRT19. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KRT19. (Distance between topics and target gene indicate popularity.) KRT19

* Data provided by citexs.com, for reference only.

Publishing research using A10948? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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