Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

VEGF Receptor 2 Rabbit mAb (A11875)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - VEGF Receptor 2 Rabbit mAb (A11875)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (A11875).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name VEGF Receptor 2 Rabbit mAb
Catalog No. A11875
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Immunogen information

Immunogen Recombinant protein of human VEGF Receptor 2
Sequence Email for sequence
Gene ID 3791
Swiss prot P35968
Synonyms FLK1; CD309; VEGFR; VEGFR2; VEGF Receptor 2
Calculated MW 152kDa
Observed MW 210kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HUVEC, HMVEC, PAE/CKR
Cellular location Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein

Research Area

VEGF Receptor 2 Rabbit mAb images

ABclonal:Western blot - VEGF Receptor 2 Rabbit mAb (A11875)}

Western blot - VEGF Receptor 2 Rabbit mAb (A11875)

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (A11875).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on KDR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KDR. (Distance between topics and target gene indicate popularity.) KDR

* Data provided by citexs.com, for reference only.

Publishing research using A11875? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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