Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IL1β Rabbit pAb (A1112)

Reviews (5) Publications (151) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - IL1β Rabbit pAb (A1112)

Western blot analysis of various lysates using IL1β Rabbit pAb (A1112) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - IL1β Rabbit pAb (A1112)

Immunofluorescence analysis of THP-1 cells using IL1β Rabbit pAb (A1112) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IL1β Rabbit pAb (A1112)

Immunofluorescence analysis of Rat spleen using IL1β Rabbit pAb (A1112) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)
Review
ABclonal: review for IL1β Rabbit pAb(A1112)

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Overview

Product name IL1β Rabbit pAb
Catalog No. A1112
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1β (NP_000567.1).
Sequence MAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLDGGIQLRISDHHYSKGFRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIFFDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
Gene ID 3553
Swiss prot P01584
Synonyms IL-1; IL1F2; IL1beta; IL1-BETA; IL1β
Calculated MW 31kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples RAW264.7, Mouse lung, Mouse liver
Cellular location Cytoplasm, Cytoplasmic vesicle, Lysosome, Secreted, autophagosome, cytosol, exosome
Customer validation

WB (Mouse embryonal fibroblast cell, HUVEC cells, Human corneal epithelial cells, Human cellsHomo sapiens, Homo sapiens, Rattus norvegicus, Mus musculus, Bos taurus, Escherichia coli, Sus scrofa, Oryctolagus cuniculus)

ELISA (Mus musculus, Homo sapiens)

IHC (Mus musculus, Rattus norvegicus)

IF (Mus musculus, Rattus norvegicus, Homo sapiens)

BAPL (Rattus norvegicus)

RT-qPCR (Homo sapiens)

IP (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

IL1β Rabbit pAb images

ABclonal:Western blot - IL1β Rabbit pAb (A1112)}

Western blot - IL1β Rabbit pAb (A1112)

Western blot analysis of various lysates using IL1β Rabbit pAb (A1112) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - IL1β Rabbit pAb (A1112)}

Immunofluorescence - IL1β Rabbit pAb (A1112)

Immunofluorescence analysis of THP-1 cells using IL1β Rabbit pAb (A1112) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL1β Rabbit pAb (A1112)}

Immunofluorescence - IL1β Rabbit pAb (A1112)

Immunofluorescence analysis of Rat spleen using IL1β Rabbit pAb (A1112) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on IL1B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IL1B. (Distance between topics and target gene indicate popularity.) IL1B

* Data provided by citexs.com, for reference only.

Publishing research using A1112? Please let us know so that we can cite the reference in this datasheet.

Antibodies (9)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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