Summary
Catalog No:A0688
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - IL17A antibody (A0688)

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Background

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).
SequenceGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA
Gene ID3605
Swiss ProtQ16552
SynonymsCTLA-8, CTLA8, IL-17, IL-17A, IL17
Calculated MW17kDa
Observed MW35kDa

Applications

ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWB
Recommended DilutionWB 1:200 - 1:500
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-937, COS7, Jurkat, THP-1, Mouse liver, Mouse heart, Mouse thymus, Rat thymus, Rat liver
Cellular LocationSecreted
Customer Validation

WB (Mouse, Rat)

IF (Mouse)

IL17A Polyclonal Antibody images

ABclonal:Western blot - IL17A antibody (A0688)

Western blot - IL17A antibody (A0688)

Western blot analysis of extracts of various cell lines, using IL17A antibody (A0688) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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