Summary
Catalog No:A1003
Host species:Mouse
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - IKBKG antibody (A1003)

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Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Immunogen information

ImmunogenA synthetic peptide of human IKBKG
SequenceEmail for sequence
Gene ID8517
Swiss ProtQ9Y6K9
SynonymsAMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
Calculated MW36kDa/48kDa/55kDa
Observed MW48kDa

Applications

ReactivityHuman
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:1000
Storage BufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, Jurkat, HT-1080, K-562
Cellular LocationCytoplasm, Nucleus
Customer Validation

WB (Human)

IKBKG Monoclonal Antibody images

ABclonal:Western blot - IKBKG antibody (A1003)

Western blot - IKBKG antibody (A1003)

Western blot analysis of extracts of various cell lines, using IKBKG antibody (A1003).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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