Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

HSP60/HSPD1 Rabbit mAb (A10906)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HSP60/HSPD1 Rabbit mAb (A10906)

Western blot analysis of various lysates using HSP60/HSPD1 Rabbit mAb (A10906).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name HSP60/HSPD1 Rabbit mAb
Catalog No. A10906
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

Immunogen information

Immunogen Recombinant protein of human Hsp60
Sequence Email for sequence
Gene ID 3329
Swiss prot P10809
Synonyms HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSP60/HSPD1
Calculated MW 61kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, MCF7, PANC-1, SW480
Cellular location Mitochondrion matrix

Documents

Certificate of Compliance

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Lot number

Research Area

HSP60/HSPD1 Rabbit mAb images

ABclonal:Western blot - HSP60/HSPD1 Rabbit mAb (A10906)}

Western blot - HSP60/HSPD1 Rabbit mAb (A10906)

Western blot analysis of various lysates using HSP60/HSPD1 Rabbit mAb (A10906).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on HSPD1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSPD1. (Distance between topics and target gene indicate popularity.) HSPD1

* Data provided by citexs.com, for reference only.

Publishing research using A10906? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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