Catalog No:A0284
Host species:Rabbit
Purity:Affinity purification
ABclonal:Western blot - HSPA1A antibody (A0284)
ABclonal:Immunofluorescence - HSPA1A antibody (A0284)

To buy,select size,quantity


This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Immunogen information

ImmunogenA synthetic peptide of human HSPA1A
SequenceEmail for sequence
Gene ID3303
Swiss ProtP0DMV8
SynonymsHEL-S-103, HSP70-1, HSP70-1A, HSP70.1, HSP70I, HSP72, HSPA1
Calculated MW70kDa
Observed MW72kDa


ReactivityHuman, Mouse
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples
Cellular Location
Customer Validation

IF (HK2, Human)

WB (HeLa, Mouse, Human, Rat)

HSPA1A Polyclonal Antibody images

ABclonal:Western blot - HSPA1A antibody (A0284)

Western blot - HSPA1A antibody (A0284)

Western blot analysis of extracts of various cell lines, using HSPA1A antibody (A0284) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - HSPA1A antibody (A0284)

Immunofluorescence - HSPA1A antibody (A0284)

Immunofluorescence analysis of U2OS cells using HSPA1A antibody (A0284). Blue: DAPI for nuclear staining.

Submit an review for A0284

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)

Reviews List

Ask Us About This Product

submit your question on A0284 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A0284? Please let us know so that we can cite the reference in this datasheet.

You Viewed