Product Type > Antibodies > Secondary Antibodies

HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Publications (1436) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Overview

Product name HRP-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No. AS014
Host species Goat
Purification method Affinity purification
Isotype Horseradish peroxidase conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rabbit IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • ELISA 1:5000 - 1:10000
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples
Cellular location
Customer validation

WB (Drosophila, Homo sapiens, Mus musculus, Rattus norvegicus, Brachyura, Oryza sativa, Crassostrea gigas, Saccharomyces cerevisiae, Caenorhabditis Elegans, Sus scrofa, Gallus gallus, Oyster, Cavia porcellus, Squaliobarbus curriculus, Oryctolagus cuniculus, Arabidopsis thaliana, M. truncatula, Malus micromalus, Tegillarca granosa, Zea mays, Drosophila melanogaster, Populus davidiana×P.bolleana, Riemerella anatipestifer, Spodoptera frugiperda, Giardia duodenalis, Ictalurus punctatus, Triticum aestivum L., Eospalax cansus, Triticum aestivum, Thale cress, Solanum tuberosum, Cryptosporidium parvum, broiler embryos, Ctenopharyngodon idellus, fenugreek, Toxoplasma gondii, Danio rerio, coli strains, Cyprinus carpio, Escherichia coli, Plutella xylostella, Haliotis, Nicotiana tabacum, Bos Taurus, Chlamys nobilis, Chlorocebus aethiops , insect, Phytophthora capsici, Anatinae, N. benthamiana, acific oyster, Hamster, C. elegans, Nicotiana benthamiana, Mesocricetus auratus, Carassius auratus gibelio, Mus musculus;Homo sapiens, Bombyx mori Linnaeus, Chlorocebus aethiops, Bos taurus, Gossypium spp, gga, Capra hircus, Chlorocebus sabaeus, Ostrinia furnacalis, Ovis aries, Sus domesticus, Carassius auratus, Ctenopharyngodon idella, A. fumigatus, Pseudosciaena crocea, Pyropia yezoensis, Zea mays L., Actinopterygii, Cuniculus, 无, Staphylococcus aureus, Ark clam, Bombyx mori, Pampus argenteus, Monkeypox virus, yeast, Tomentosa, , Yellow catfish, homo sapiens, AgnusDei, Homo sapiensRattus norvegicus, Rice, Rabbit, Mytilus coruscus, S. cerealella, Homo sapiensMus musculus, mus musculus, A. fumigatus strains, Leporidae, Anser cygnoides, ovis aries, Misgurnus anguillicaudatus, Cabbage beetles, Xenopus laevis, Other, immortalized porcine alveolar macrophage (iPAM) cells)

IHC (Homo sapiens, Scophthalmus maximus, Mus musculus, Rattus norvegicus, Bos taurus, Capra hircus, Cervus nippon, Misgurnus anguillicaudatus, Oryctolagus cuniculus)

ICC (Rattus norvegicus, Mus musculus, Homo sapiens)

IP (Mus musculus, Homo sapiens)

IF (Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Felis catus, Capra hircus, Homo sapiensMus musculus, Oryctolagus cuniculus)

ELISA (Grass carp, Rattus norvegicus, Zea mays, Carassius auratus gibelio, Sus scrofa, Crassostrea gigas, Mus musculus, SARS-CoV-2, Homo sapiens)

BrdU (Homo sapiens)

PAMPs binding assa (OystersC. gigas)

MTT (Pleuronectiformes)

FC (Homo sapiens)

co-ip (Homo sapiens)

PCR (Homo sapiens)

PE (Mus musculus)

Cellular Uptake and In Vitro Fibrotic Marker Visual-ization (Mus musculus)

DB (Homo sapiens)

IB (Coniothyrium minitans)

WBIHC (Gallus gallus)

CO-IP (Homo sapiens, Mus musculus)

RT-PCR (Homo sapiens, Mus musculus)

IHC/IF (mus musculus)

Elisa (Homo sapiens)

Co-IP (Xenopus laevis)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

HRP-conjugated Goat anti-Rabbit IgG (H+L) images

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Goat Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS014? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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