Product Type > Antibodies > Tag and Loading Control Antibodies

HRP-conjugated β-Tubulin Mouse mAb (AC030)

Publications (14) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HRP-conjugated β-Tubulin Mouse mAb (AC030)

Western blot analysis of various lysates using HRP-conjugated β-Tubulin Mouse mAb (AC030) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name HRP-conjugated β-Tubulin Mouse mAb
Catalog No. AC030
Host species Mouse
Purification method Affinity purification
Isotype IgG1, Kappa
CloneNo. AMC0499

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen information

Immunogen Recombinant protein of human TUBB
Sequence Email for sequence
Gene ID 203068
Swiss prot P07437
Synonyms M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; HRP-conjugated β-Tubulin
Calculated MW 50kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:10000 - 1:50000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, U-87MG, 293T, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular location Cytoplasm, cytoskeleton
Customer validation

WB (Rattus norvegicus, Homo sapiens, Drosophila melanogaster, Mus musculus)

IHC (Homo sapiens)

IP (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

HRP-conjugated β-Tubulin Mouse mAb images

ABclonal:Western blot - HRP-conjugated β-Tubulin Mouse mAb (AC030)}

Western blot - HRP-conjugated β-Tubulin Mouse mAb (AC030)

Western blot analysis of various lysates using HRP-conjugated β-Tubulin Mouse mAb (AC030) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on TUBB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TUBB. (Distance between topics and target gene indicate popularity.) TUBB

* Data provided by citexs.com, for reference only.

Publishing research using AC030? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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