Product Type > Antibodies > Tag and Loading Control Antibodies

HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein , 293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with NR3C2-C Protein, 293F transfected with WDR77-C Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name HRP-conjugated Mouse anti HA-Tag mAb
Catalog No. AE025
Host species Mouse
Purification method Affinity purification
Isotype IgG1, Kappa
CloneNo. AMC0557

Background

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Immunogen information

Immunogen A synthetic peptide corresponding to HA tag.
Sequence YPYDVPDYA
Gene ID
Swiss prot
Synonyms HA; HA tag; HA-tag
Calculated MW
Observed MW 53kDa(CD95)/65kDa(CD2)/53kDa(SCRN1)/26KD(HA(N端)/42kDa(WDR77)/45kDa(NR3C2)

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T-CD2-3*HA(N), 293T-CD2-3*HA(C), 293F, 293F-CD95-3*HA(N), 293F-CD95-3*HA(C)293F-WDR77-HA-GFP(C), 293T-SCRN1-HA-GFP(N), 293T-SCRN1-HA-GFP(C), 293T-HA(N)
Cellular location
Customer validation

WB (Mus musculus)

IP (Mus musculus)

Documents

Certificate of Compliance

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Lot number

HRP-conjugated Mouse anti HA-Tag mAb images

ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)}

Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)}

Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein , 293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)}

Western blot - HRP-conjugated Mouse anti HA-Tag mAb (AE025)

Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with NR3C2-C Protein, 293F transfected with WDR77-C Protein using HRP-conjugated Mouse anti HA-Tag mAb (AE025) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on HA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HA. (Distance between topics and target gene indicate popularity.) HA

* Data provided by citexs.com, for reference only.

Publishing research using AE025? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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