Product Type > Antibodies > Tag and Loading Control Antibodies

HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Publications (8) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Species independent

ABclonal:Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Western blot analysis of lysates from 293F cells transfected with IRF1-Flag protein , using HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Western blot analysis of lysates from 293T cells transfected with GSK3B-Flag protein using HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GSK3B using HRP-conjugated Mouse anti DDDDK-Tag mAb(AE024) at 1:1000 dilution. 
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.1s.

You may also interested in:

Overview

Product name HRP-conjugated Mouse anti DDDDK-Tag mAb
Catalog No. AE024
Host species Mouse
Purification method Affinity purification
Isotype IgG
CloneNo. AMC0382-HRP

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Immunogen information

Immunogen A synthetic peptide corresponding to DDDDK tag.
Sequence DYKDDDDK
Gene ID
Swiss prot
Synonyms DDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW 50kDa/46kDa/48kDa

Applications

Reactivity Species independent
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:6000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3
Application key Western blotting    
Positive samples 293T-GSK3B-Flag(C-terminal), 293F-IRF1-Flag(3*N-terminal), 293T
Cellular location
Customer validation

Elisa (Paralichthys olivaceus)

WB (Mus musculus, Saccharomyces cerevisiae, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

HRP-conjugated Mouse anti DDDDK-Tag mAb images

ABclonal:Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)}

Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Western blot analysis of lysates from 293F cells transfected with IRF1-Flag protein , using HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)}

Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Western blot analysis of lysates from 293T cells transfected with GSK3B-Flag protein using HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)}

Western blot - HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GSK3B using HRP-conjugated Mouse anti DDDDK-Tag mAb(AE024) at 1:1000 dilution. 
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.1s.

Inquire About This Product

Submit your question about AE024 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on DDDDK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DDDDK. (Distance between topics and target gene indicate popularity.) DDDDK

* Data provided by citexs.com, for reference only.

Publishing research using AE024? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order