Product Type > Antibodies > Tag and Loading Control Antibodies

HRP-conjugated β-Actin Rabbit mAb (AC028)

Publications (45) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of various lysates using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:50000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:1000- 1:512000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name HRP-conjugated β-Actin Rabbit mAb
Catalog No. AC028
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC5115-01

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.

Immunogen information

Immunogen Recombinant protein of human HRP-conjugated β-Actin
Sequence Email for sequence
Gene ID 60
Swiss prot P60709
Synonyms BRWS1; PS1TP5BP1; HRP-conjugated β-Actin
Calculated MW 42kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat, Chicken, Zebrafish, Pig
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:2000 - 1:50000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293F, HeLa, HepG2, NIH/3T3, Mouse brain, C6
Cellular location Cytoplasm, cytoskeleton
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Gallus gallus, Aves, Oncorhynchus mykiss, Drosophila melanogaster, Zea mays)

Documents

Certificate of Compliance

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Lot number

Research Area

HRP-conjugated β-Actin Rabbit mAb images

ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)}

Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of various lysates using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:50000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)}

Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:1000- 1:512000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC028? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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