Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

hnRNP K Rabbit pAb (A1701)

Reviews (4) Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - hnRNP K Rabbit pAb (A1701)

Western blot analysis of extracts of various cell lines, using hnRNP K antibody (A1701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - hnRNP K Rabbit pAb (A1701)

Immunohistochemistry analysis of paraffin-embedded rat liver using hnRNP K Antibody (A1701) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - hnRNP K Rabbit pAb (A1701)

Immunohistochemistry analysis of paraffin-embedded human esophagus using hnRNP K Antibody (A1701) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - hnRNP K Rabbit pAb (A1701)

Immunohistochemistry analysis of paraffin-embedded mouse esophagus using hnRNP K Antibody (A1701) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - hnRNP K Rabbit pAb (A1701)

Immunofluorescence analysis of MCF-7 cells using hnRNP K antibody (A1701). Blue: DAPI for nuclear staining.

Review
ABclonal: review for hnRNP K Rabbit pAb(A1701)
Review
ABclonal: review for hnRNP K Rabbit pAb(A1701)
Review
ABclonal: review for hnRNP K Rabbit pAb(A1701)
Review
ABclonal: review for hnRNP K Rabbit pAb(A1701)

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Overview

Product name hnRNP K Rabbit pAb
Catalog No. A1701
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human hnRNP K (NP_112552.1).
Sequence METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDY
Gene ID 3190
Swiss prot P61978
Synonyms AUKS; CSBP; TUNP; HNRPK; hnRNP K
Calculated MW 51kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, Jurkat, 293T, MCF7, HepG2, PC-12, Mouse testis
Cellular location Cell projection, Cytoplasm, Nucleus, nucleoplasm, podosome
Customer validation

IP (MESC, 293T)

WB (293T, Mouse CD4T cellsHomo sapiens, Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

hnRNP K Rabbit pAb images

ABclonal:Western blot - hnRNP K Rabbit pAb (A1701)}

Western blot - hnRNP K Rabbit pAb (A1701)

Western blot analysis of extracts of various cell lines, using hnRNP K antibody (A1701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - hnRNP K Rabbit pAb (A1701)}

Immunohistochemistry - hnRNP K Rabbit pAb (A1701)

Immunohistochemistry analysis of paraffin-embedded rat liver using hnRNP K Antibody (A1701) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - hnRNP K Rabbit pAb (A1701)}

Immunohistochemistry - hnRNP K Rabbit pAb (A1701)

Immunohistochemistry analysis of paraffin-embedded human esophagus using hnRNP K Antibody (A1701) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - hnRNP K Rabbit pAb (A1701)}

Immunohistochemistry - hnRNP K Rabbit pAb (A1701)

Immunohistochemistry analysis of paraffin-embedded mouse esophagus using hnRNP K Antibody (A1701) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - hnRNP K Rabbit pAb (A1701)}

Immunofluorescence - hnRNP K Rabbit pAb (A1701)

Immunofluorescence analysis of MCF-7 cells using hnRNP K antibody (A1701). Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on HNRNPK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HNRNPK. (Distance between topics and target gene indicate popularity.) HNRNPK

* Data provided by citexs.com, for reference only.

Publishing research using A1701? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (1)

Antibodies (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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