Summary
Catalog No:A1346
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - HMOX1 antibody (A1346)

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Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Immunogen information

ImmunogenRecombinant protein of human HMOX1
SequenceEmail for sequence
Gene ID3162
Swiss ProtP09601
SynonymsbK286B10, HMOX1D, HO-1, HSP32
Calculated MW32kDa
Observed MW33kDa

Applications

ReactivityHuman, Mouse
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Mouse spleen
Cellular LocationCytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein
Customer Validation

WB (Mouse liver, Rat)

HMOX1 Polyclonal Antibody images

ABclonal:Western blot - HMOX1 antibody (A1346)

Western blot - HMOX1 antibody (A1346)

Western blot analysis of extracts of various cell lines, using HMOX1 antibody (A1346) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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