Summary
Catalog No:A2553
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - HMGB1 antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)
ABclonal:Immunofluorescence - HMGB1 antibody (A2553)

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Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
SequenceSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE
Gene ID3146
Swiss ProtP09429
SynonymsHMG-1, HMG1, HMG3, SBP-1
Calculated MW24kDa
Observed MW26kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF-7, HL-60, SW480, Raji, Mouse spleen, Mouse lung, Rat lung
Cellular LocationCell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted
Customer Validation

WB (Mouse brain, Mouse spinal cord, Human)

IHC (HeLa)

HMGB1 Polyclonal Antibody images

ABclonal:Western blot - HMGB1 antibody (A2553)

Western blot - HMGB1 antibody (A2553)

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (A2553) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human amygdalitis using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human stomach using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human adenomyosis using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded rat lung using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human stomach using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human gastric cancer using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded human adenomyosis using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded mouse lung using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded mouse liver using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded mouse brain using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HMGB1 Antibody (A2553)

Immunohistochemistry - HMGB1 Antibody (A2553)

Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (A2553) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - HMGB1 antibody (A2553)

Immunofluorescence - HMGB1 antibody (A2553)

Immunofluorescence analysis of HeLa cells using HMGB1 antibody (A2553) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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