Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

HLA-G Mouse mAb (A10182)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - HLA-G Mouse mAb (A10182)

Western blot analysis of lysates from HL60 cells, using HLA-G Mouse mAb (A10182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name HLA-G Mouse mAb
Catalog No. A10182
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

Immunogen information

Immunogen Recombinant protein of human HLA-G
Sequence Email for sequence
Gene ID 3135
Swiss prot P17693
Synonyms MHC-G; HLA-G
Calculated MW 38kDa
Observed MW 50kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples HL-60
Cellular location Membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

HLA-G Mouse mAb images

ABclonal:Western blot - HLA-G Mouse mAb (A10182)}

Western blot - HLA-G Mouse mAb (A10182)

Western blot analysis of lysates from HL60 cells, using HLA-G Mouse mAb (A10182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on HLA-G. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HLA-G. (Distance between topics and target gene indicate popularity.) HLA-G

* Data provided by citexs.com, for reference only.

Publishing research using A10182? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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