Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

HDAC3 Rabbit pAb (A2139)

Publications (9) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HDAC3 Rabbit pAb (A2139)

Western blot analysis of extracts of various cell lines, using HDAC3 antibody (A2139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded human colon using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded mouse liver using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded rat ovary using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of HeLa cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of K-562 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of PC-12 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of U2OS cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of HeLa cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of K-562 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of PC-12 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of U2OS cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name HDAC3 Rabbit pAb
Catalog No. A2139
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 299-428 of human HDAC3 (NP_003874.2).
Sequence TVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI
Gene ID 8841
Swiss prot O15379
Synonyms HD3; RPD3; KDAC3; RPD3-2; HDAC3
Calculated MW 49kDa
Observed MW 49kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, MCF7, NIH/3T3, C6
Cellular location Cytoplasm, Nucleus, cytosol
Customer validation

WB (Homo sapiens, Mus musculus)

IF (Gallus gallus, Homo sapiens)

Co-IP (Rattus norvegicus)

ChIP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

HDAC3 Rabbit pAb images

ABclonal:Western blot - HDAC3 Rabbit pAb (A2139)}

Western blot - HDAC3 Rabbit pAb (A2139)

Western blot analysis of extracts of various cell lines, using HDAC3 antibody (A2139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)}

Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)}

Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded human colon using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)}

Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded mouse liver using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HDAC3 Rabbit pAb (A2139)}

Immunohistochemistry - HDAC3 Rabbit pAb (A2139)

Immunohistochemistry analysis of paraffin-embedded rat ovary using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of HeLa cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of K-562 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of PC-12 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of U2OS cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of HeLa cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of K-562 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of PC-12 cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - HDAC3 Rabbit pAb (A2139)}

Immunofluorescence - HDAC3 Rabbit pAb (A2139)

Immunofluorescence analysis of U2OS cells using HDAC3 Rabbit pAb (A2139) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on HDAC3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HDAC3. (Distance between topics and target gene indicate popularity.) HDAC3

* Data provided by citexs.com, for reference only.

Publishing research using A2139? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

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