Summary
Catalog No:A0238
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - HDAC1 antibody (A0238)
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)
ABclonal:Immunofluorescence - HDAC1 antibody (A0238)

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Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Immunogen information

ImmunogenA synthetic peptide of human HDAC1
SequenceEmail for sequence
Gene ID3065
Swiss ProtQ13547
SynonymsGON-10, HD1, RPD3, RPD3L1
Calculated MW55kDa
Observed MW60kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:100
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationNucleus
Customer Validation

IF (Human acute leukemia cells)

WB (Human acute leukemia cells, HL60,Hut102, 293T, Rat)

IP (Human acute leukemia cells, Rat)

ChIP (Rat)

HDAC1 Polyclonal Antibody images

ABclonal:Western blot - HDAC1 antibody (A0238)

Western blot - HDAC1 antibody (A0238)

Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A0238).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)

Immunohistochemistry - HDAC1 antibody (A0238)

Immunohistochemistry of paraffin-embedded human lung cancer using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)

Immunohistochemistry - HDAC1 antibody (A0238)

Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)

Immunohistochemistry - HDAC1 antibody (A0238)

Immunohistochemistry of paraffin-embedded human esophagus using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - HDAC1 antibody (A0238)

Immunohistochemistry - HDAC1 antibody (A0238)

Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - HDAC1 antibody (A0238)

Immunofluorescence - HDAC1 antibody (A0238)

Immunofluorescence analysis of A549 cells using HDAC1 antibody (A0238). Blue: DAPI for nuclear staining.

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