Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KD Validated] HDAC1 Rabbit pAb (A0238)

Reviews (5) Publications (18) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)

Western blot analysis of various lysates using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:2000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 20 s.

ABclonal:Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)

Western blot analysis of lysates from wild type (WT) and HDAC1 knockdown (KD) 293T cells, using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of HDAC1 in paraffin-embedded rat spleen using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human lung cancer using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human colon using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunofluorescence analysis of A549 cells using [KD Validated] HDAC1 Rabbit pAb (A0238). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)

Confocal immunofluorescence analysis of L929 cells using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg [KD Validated] HDAC1 Rabbit pAb (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000.

Review
ABclonal: review for [KD Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KD Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KD Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KD Validated] HDAC1 Rabbit pAb(A0238)
Review
ABclonal: review for [KD Validated] HDAC1 Rabbit pAb(A0238)

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Overview

Product name [KD Validated] HDAC1 Rabbit pAb
Catalog No. A0238
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 393-482 of human HDAC1 (NP_004955.2).
Sequence SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA
Gene ID 3065
Swiss prot Q13547
Synonyms HD1; RPD3; KDAC1; GON-10; RPD3L1; C1
Calculated MW 55kDa
Observed MW 62kDa/

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples 293T, HeLa, NIH/3T3
Cellular location Nucleus
Customer validation

IF (Human acute leukemia cells)

WB (Human acute leukemia cells, HL60,Hut102, 293THomo sapiens, Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus)

IP (Human acute leukemia cellsRattus norvegicus)

ChIP (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] HDAC1 Rabbit pAb images

ABclonal:Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)

Western blot analysis of various lysates using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:2000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 20 s.
ABclonal:Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)

Western blot analysis of lysates from wild type (WT) and HDAC1 knockdown (KD) 293T cells, using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of HDAC1 in paraffin-embedded rat spleen using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human lung cancer using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human colon using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunofluorescence analysis of A549 cells using [KD Validated] HDAC1 Rabbit pAb (A0238). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)

Confocal immunofluorescence analysis of L929 cells using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KD Validated] HDAC1 Rabbit pAb (A0238)}

Immunoprecipitation - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg [KD Validated] HDAC1 Rabbit pAb (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on HDAC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HDAC1. (Distance between topics and target gene indicate popularity.) HDAC1

* Data provided by citexs.com, for reference only.

Publishing research using A0238? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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