Product Type > Antibodies > Tag and Loading Control Antibodies

GAPDH Rabbit pAb (AC001)

Review (1) Publications (404) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded rat kidney using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded rat pancreas using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded human liver cancer using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded mouse lung using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of PC-12 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of HeLa cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - GAPDH Rabbit pAb (AC001)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg GAPDH antibody (AC001). Western blot was performed from the immunoprecipitate using GAPDH antibody (AC001) at a dilution of 1:20000.

Review
ABclonal: review for GAPDH Rabbit pAb(AC001)

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Overview

Product name GAPDH Rabbit pAb
Catalog No. AC001
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
Sequence MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID 2597
Swiss prot P04406
Synonyms G3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW 36kDa
Observed MW

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:10000 - 1:30000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa, 293T, Hep G2, Mouse liver, Rat liver
Cellular location Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB (MSC cellsHomo sapiens, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Gallus gallus, Danio rerio, gga, Ctenopharyngodon idellus, Paralichthysolivaceus, Cyprinus carpio, Cobitis taenia Linnaeus, homo sapiens, Ovis aries, Cryptosporidium parvum, Chicken, Capra hircus, Misgurnus anguillicaudatus, Other, Bos taurus)

Co-IP (Arabidopsis thaliana, Homo sapiens)

ELISA (Mus musculus)

IF (Gallus gallus, Mus musculus, Rattus norvegicus, Homo sapiens)

RT-qPCR (Mus musculus)

qRT-PCR (Gallus gallus, Homo sapiens)

qPCR (Homo sapiens)

Hcp protein secretion assay (aeromonas hydrophila)

IHC (Mus musculus, Rattus norvegicus, Homo sapiens, Gallus gallus)

IP (Gallus gallus)

Documents

Certificate of Compliance

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Lot number

Research Area

GAPDH Rabbit pAb images

ABclonal:Western blot - GAPDH Rabbit pAb (AC001)}

Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - GAPDH Rabbit pAb (AC001)}

Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded rat kidney using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded rat pancreas using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded human liver cancer using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of GAPDH in paraffin-embedded mouse lung using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of PC-12 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of HeLa cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - GAPDH Rabbit pAb (AC001)}

Immunoprecipitation - GAPDH Rabbit pAb (AC001)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg GAPDH antibody (AC001). Western blot was performed from the immunoprecipitate using GAPDH antibody (AC001) at a dilution of 1:20000.

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Related Targets

Based on existing publications, the following genes are closely related to research on GAPDH. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GAPDH. (Distance between topics and target gene indicate popularity.) GAPDH

* Data provided by citexs.com, for reference only.

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