Summary
Catalog No:AC001
Host species:Rabbit
Purity:Affinity purification
Isotype:IgG
ABclonal:Western blot - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)
ABclonal:Immunofluorescence - GAPDH antibody (AC001)

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Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen information

ImmunogenRecombinant protein of human GAPDH
SequenceEmail for sequence
Gene ID2597
Swiss ProtP04406
SynonymsG3PD, GAPD, HEL-S-162eP
Calculated MW31kDa/36kDa
Observed MW36kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:1000 - 1:10000
IHC 1:50 - 1:100
IF 1:20 - 1:50
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular LocationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer Validation

WB (Mouse, Human, Rat)

GAPDH Polyclonal Antibody images

ABclonal:Western blot - GAPDH antibody (AC001)

Western blot - GAPDH antibody (AC001)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded rat pancreas using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded human breast cancer using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry  - GAPDH antibody (AC001)

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH antibody (AC001)

Immunofluorescence - GAPDH antibody (AC001)

Immunofluorescence analysis of MCF-7 cells using GAPDH antibody (AC001). Blue: DAPI for nuclear staining.

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